Lantana camara plant named &#39;uf-1013-1&#39;

ABSTRACT

A new and distinct cultivar of  Lantana camara  plant named ‘UF-1013-1’, characterized by its moderate vigor, mounding growth habit, dense branches, round plant form and canopy, free flowering, bright yellow and red flowers, no to little fruiting, no to few seeds, a very high level of female infertility, a very low level of pollen stainability and viability, a very high level of male infertility, lack of hybridization with  Lantana depressa,  triploidy and a unique combination of DNA marker alleles, is disclosed.

CROSS-REFERENCE TO RELATED APPLICATIONS

This Application is related to and claims the benefit of U.S.Provisional Patent Application Ser. No. 62/946,666, filed on Dec. 11,2019, entitled LANTANA CAMERA PLANT NAMED ‘UF-1013-1’, the entirety ofwhich is incorporated herein by reference.

ACKNOWLEDGEMENT OF FEDERAL RESEARCH SUPPORT

This invention was made with government support under FLA-GCR-005065 andFLA-GCR-005507 awarded by the National Institute of Food andAgriculture, USDA, the USDA/Tropical and Subtropical AgricultureResearch (TSTAR) program, and the Florida Department of Agriculture andConsumer Service (FDACS) Specialty Crop Block Grant program. The UnitedStates government has certain rights in the invention.

Genus and species: Lantana camara L. (Lantana strigocamara R. W.Sanders).

Cultivar denomination: The present disclosure relates to Lantana camaracultivar ‘UF-1013-1’.

BACKGROUND OF THE NEW CULTIVAR

The present invention relates to a new and distinct cultivar of lantana,botanically known as Lantana camara, and hereinafter referred to by thename ‘UF-1013-1’. Lantana camara (L. camara) is a member of the familyVerbenaceae. Plants of this species produce brightly-colored flowers,attract butterflies, tolerate harsh environmental conditions, have lowmaintenance requirements, and are easy to grow. Plants of L. camara arewidely used in containers, hanging baskets, and landscapes. Commercialproduction of L. camara is widespread in the nursery industry,especially in the southern United States.

Lantana camara is native to Central and South Americas including theWest Indies and was introduced to the United States in the 1800s. Sincethen, L. camara has escaped cultivation and become naturalized inthirteen U.S. states. In Florida, escaped L. camara has hybridized withLantana depressa, a small and endangered Florida native species. Lantanacamara has been listed as a Category I invasive species in Florida bythe Florida Exotic Pest Plant Council (FLEPPC) (FLEPPC Invasive PlantLists, 2019). The Georgia Exotic Pest Plant Council (GA-EPPC) has listedL. camara as a Category 3 exotic plant, a minor problem in the Georgia'snatural areas or not yet known to be a problem in Georgia but known tobe problem in adjacent states (GA-EPPC List of Non-native InvasivePlants in Georgia, 2019). There has been a strong need for thedevelopment of new infertile cultivars in L. camara to protect theenvironment and the native species and provide new introductions for thenursery and landscape industries.

The new lantana cultivar ‘UF-1013-1’ is a product of a planned breedingprogram at the University of Florida's Gulf Coast Research and EducationCenter (UF/GCREC) in Balm, Fla. The primary objective of the breedingprogram is to develop new infertile lantana cultivars with desirableplant stature, dense branching habits, and attractive flower colors.

The new lantana cultivar ‘UF-1013-1’ resulted from a planned crossbetween a proprietary breeding line DROP-25 (female parent) and cultivar‘Landmark Flame Improved’ (male parent; ‘Balandimfla’, U.S. Plant patentapplication Ser. No. 11/015,489, now abandoned). The stated cross wasmade in fall 2010 in Balm, Fla. The new lantana cultivar was discoveredand selected in Balm, Fla. in April 2012 as one flowering plant withinthe progeny of the stated cross.

Asexual propagation of the new lantana cultivar by vegetative cuttingsin a controlled environment in Balm, Fla. since 2012 has shown that theunique features of this new lantana cultivar are stable and reproducetrue to type plants in successive generations.

Plant Breeder's Rights for this cultivar have not been applied for. Thenew lantana cultivar ‘UF-1013-1’ has not been made publicly availablemore than one year prior to the filing of this application.

SUMMARY OF THE INVENTION

The new lantana cultivar has not been observed under all possibleenvironmental conditions. The phenotype of the new cultivar may varywith variations in environment and cultural practices such astemperature, light intensity, shading, tipping, pruning, fertilization,irrigation, and application of plant growth regulators without anychange in genotype.

The following traits have been repeatedly observed and are determined tobe the unique characteristics of the new lantana cultivar. Thesecharacteristics in combination distinguish ‘UF-1013-1’ as a new anddistinct cultivar of Lantana camara:

-   -   1. moderate plant vigor;    -   2. mounding and partially upwardly and spreading growth habit;    -   3. very dense branching;    -   4. compact plant form and canopy;    -   5. free flowering;    -   6. yellow- and red-colored flowers;    -   7. production of full, attractive plants in containers;    -   8. little fruiting and no or few berries, little seed        production, and high level of female infertility;    -   9. aborted pollen and very low pollen stainability;    -   10. no to little hybridization potential with Lantana depressa;    -   11. approximately 4.82 picograms of nuclear DNA content in        triploid somatic cells and tissues; and    -   12. carrying two alleles (152 and 160 base pairs (bp)) at the        Lantana11 simple sequence repeat (SSR) marker locus, three        alleles (135, 143 and 147 bp) at the Lantana12 SSR marker locus,        and one allele (93 bp) at the Lantana20 SSR marker locus.

Plants of the new Lantana camara cultivar ‘UF-1013-1’ differ from plantsof the female parent, DROP-25, in the following characteristics:

-   -   1. plants of the new cultivar ‘UF-1013-1’ are triploids, while        plants of DROP-25 are tetraploids;    -   2. plants of the new cultivar ‘UF-1013-1’ are mounding and have        dense branches, while plants of DROP-25 have few branches and an        erratic branching habit;    -   3. plants of the new cultivar ‘UF-1013-1’ are more vigorous and        taller than plants of DROP-25;    -   4. plants of the new cultivar ‘UF-1013-1’ produce no or few        fruit, no or few seeds, and are highly female-infertile, while        plants of DROP-25 are more female-fertile and produce more        fruit;    -   5. plants of the new cultivar ‘UF-1013-1’ have very low pollen        stainability or viability, while plants of DROP-25 have higher        pollen stainability or viability; and    -   6. plants of the new cultivar ‘UF-1013-1’ carry two alleles (152        and 160 bp) at the Lantana11 marker locus, three alleles (135,        143 and 147 bp) at the Lantana12 marker locus and one allele (93        bp) at the Lantana20 marker locus, while plants of DROP-25 carry        three alleles (150, 152 and 160 bp) at the Lantana11 marker        locus, four alleles (135, 143, 145 and 147 bp) at the Lantana12        marker locus, and two alleles (93 and 109 bp) at the Lantana20        marker locus.

Plants of the new cultivar ‘UF-1013-1’ differ from plants of the maleparent, ‘Landmark Flame Improved’, in the following characteristics:

-   -   1. plants of the new cultivar ‘UF-1013-1’ are triploids, while        plants of ‘Landmark Flame Improved’ are diploids;    -   2. plants of the new cultivar ‘UF-1013-1’ are mounding and have        a round form and canopy, while plants of ‘Landmark Flame        Improved’ are much larger, spreading, and have an open canopy;    -   3. flowers of the new cultivar ‘UF-1013-1’ are yellow-colored        when initially open and turn red when matured, while flowers of        ‘Landmark Flame Improved’ are gold to orange;    -   4. plants of the new cultivar ‘UF-1013-1’ produce no or few        fruit or seeds and are highly female-infertile, while plants of        ‘Landmark Flame Improved’ are female-fertile and produce more        fruit and seeds; and    -   5. Plants of the new cultivar ‘UF-1013-1’ have low pollen        stainability or viability, while plants of ‘Landmark Flame        Improved’ have higher pollen stainability or viability.

Plants of the new Lantana camara cultivar ‘UF-1013-1’ differ from plantsof its sibling cultivar, ‘UF-1013A-2A’ (Bloomify™ Red), U.S. Plant Pat.No. 29,292), in the following characteristics:

-   -   1. plants of the new cultivar ‘UF-1013-1’ are more compact than        plants of ‘UF-1013A-2A’;    -   2. plants of the new cultivar ‘UF-1013-1’ have a more spreading        canopy than plants of ‘UF-1013A-2A’;    -   3. flowers of the new cultivar ‘UF-1013-1’ are larger than        plants of ‘UF-1013A-2A’;    -   4. flowers of the new cultivar ‘UF-1013-1’ have a darker red        color than plants of ‘UF-1013A-2A’;    -   5. plants of the new cultivars ‘UF-1013-1’ have a higher nuclear        DNA content than plants of ‘UF-1013A-2A’ (4.8 pg/2C vs. 4.5        pg/2C in ‘UF-1013A-2A’);    -   6. plants of the new cultivar ‘UF-1013-1’ carry two alleles (135        and 147 bp) at the Lantana12 marker locus, while plants of        ‘UF-1013A-2A’ miss these two alleles.

Plants of the new lantana cultivar ‘UF-1013-1’ can also be compared tothe cultivar ‘Landscape Bandana Red Improved’ (commercial cultivar, notpatented). In side-by-side comparisons conducted in Balm, Fla., plantsof the new lantana cultivar differed from plants of ‘Landscape BandanaRed Improved’ in the following characteristics:

-   -   1. plants of the new cultivar ‘UF-1013-1’ are shorter and        narrower and more compact, while plants of ‘Landscape Bandana        Red Improved’ are taller and wider;    -   2. plants of the new cultivar ‘UF-1013-1’ form a compact,        upright canopy in containers, while plants of ‘Landscape Bandana        Red Improved’ have drooping branches and form a very open        canopy;    -   3. inflorescences of the new cultivar ‘UF-1013-1’ are larger        than plants of ‘Landscape Bandana Red Improved’; and    -   4. mature flowers of the new cultivar ‘UF-1013-1’ are much        redder, while the mature flowers of ‘Landscape Bandana Red        Improved’ are light pink to salmon.

Plants of the new lantana cultivar ‘UF-1013-1’ can be compared to‘Luscious® Citrus Blend’ (‘Balandusbi’, U.S. Plant Pat. No. 32,018). Inside-by-side comparisons conducted in Balm, Fla., plants of the newlantana cultivar differed from plants of ‘Luscious® Citrus Blend’ in thefollowing characteristics:

-   -   1. Plants of the new cultivar ‘UF-1013-1’ are narrower and more        compact, while plants of ‘Luscious® Citrus Blend’ are wider;    -   2. Plants of the new cultivar ‘UF-1013-1’ are more upright,        while plants of ‘Luscious® Citrus Blend’ are more spreading and        open;    -   3. Mature flowers of the new cultivar ‘UF-1013-1’ are red in        color, while mature flowers of ‘Luscious® Citrus Blend’ are gold        and orange in color; and    -   4. Plants of the new cultivar ‘UF-1013-1’ are highly sterile and        produce little fruit, while plants of ‘Luscious® Citrus Blend’        produce much more fruit and have much higher levels of        fertility.

DESCRIPTION OF THE FIGURES

The accompanying photographs (as shown in FIGS. 1-7) illustrate theoverall appearance of the new Lantana camara cultivar ‘UF-1013-1’. Thesephotographs show the colors as true as can be reasonably obtained incolored reproductions of this type. Colors in the photographs may differslightly from the color values cited in the detailed botanicaldescription, which accurately describe the colors of the new lantanacultivar.

FIG. 1 shows a side perspective view of a typical flowering plant of thenew lantana cultivar ‘UF-1013-1’ to show its growth and branching habit,plant form, and flower color. The plant was grown in a gallon (16.5 cmin diameter and 16 cm tall) container and is shown at approximately 16weeks old. The plant was propagated from cuttings and pinched once;

FIG. 2 shows a top view of a typical flowering plant of the new lantanacultivar ‘UF-1013-1’ to show its growth and branching habit, plant form,and flower color. The plant was grown in a gallon (16.5 cm in diameterand 16 cm tall) container and is shown at approximately 16 weeks old.The plant was propagated from cuttings and pinched once;

FIG. 3 shows a side perspective view of breeding line DROP-25 (left)(female parent) and the new lantana cultivar ‘UF-1013-1’ (right) whenthey were grown in gallon (16.5-cm diameter) containers. They wereapproximately 16 weeks old grown from rooted cuttings that were pinchedonce;

FIG. 4 shows a side perspective view of cultivar ‘UF-1013A-2A’ (left)and the new cultivar ‘UF-1013-1’ (right) when they were grown in gallon(16.5-cm diameter) containers. They were approximately 16 weeks old andgrown from rooted cuttings that were pinched once;

FIG. 5 shows a top view of fully open flowers of cultivar ‘UF-1013A-2A’(left) and the new cultivar ‘UF-1013-1’ (right). The flowers were takenfrom plants of these cultivars grown in the ground bed in full sun inCitra, Fla.;

FIG. 6 shows a side perspective view of the new cultivar ‘UF-1013-1’(left) and ‘Landscape Bandana Red Improved’ (commercial cultivar, notpatented) (right) when they were grown in gallon (16.5-cm diameter)containers. They were approximately 16 weeks old and grown from rootedcuttings that were pinched once;

FIG. 7 shows a side perspective view of ‘Luscious® Citrus Blend’ (left)and the new cultivar ‘UF-1013-1’ (right) when they were grown in gallon(16.5-cm diameter) containers. They were approximately 16 weeks old andgrown from rooted cuttings that were pinched once.

DETAILED BOTANICAL DESCRIPTION OF THE CULTIVAR

In the following description, color references are made to The RoyalHorticultural Society (R.H.S.) Colour Chart, 1986 Edition, except wheregeneral terms of ordinary dictionary significance are used.

Description of Growing Conditions

Plants used for the description were grown in the fall and winter of2019 and early spring of 2020 in Balm, Fla. for 24 weeks from whenterminal cuttings were made. Cuttings were dipped into a commercialrooting hormone solution (Dip & Grow®, Dip'N Grow, Inc., Clackamas,Oreg.) on 30 August 2019; rooted cuttings were potted up in galloncontainers on 26 Sep. 2019. Plants in gallon containers were pinched on10 Oct. 2019. Plants were grown in the greenhouse until 24 Feb. 2020.Plant data was taken and plant, foliage, and flower descriptions weremade. During the production of the plants in the greenhouse,temperatures ranged from about 22.2° C. to about 35.5° C.

Botanical Description

-   Botanical classification:    -   -   Family.—Verbenaceae.        -   Botanical name.—Lantana camara (L. camara).        -   Common name.—Lantana.        -   Cultivar.—‘UF-1013-1’.-   Parentage:    -   -   Female or seed parent.—DROP-25.        -   Male or pollen parent.—‘Landmark Flame Improved’.-   Propagation:    -   -   Type.—Terminal cutting.        -   Time to initiate roots.—About nine days at 27° C.        -   Time to produce rooted cuttings.—About 25 days at 27° C.-   Root description:    -   -   Type.—Fine, fibrous; initially glaucous white in color.        -   Color.—Close to white (RHS 155B).        -   Rooting habit.—Freely branching.-   Plant description:    -   -   Plant form.—Flowering subshrub.        -   Growth habit.—Outwardly spreading and partially upright            plant habit; mounded plant form and canopy; dense branching;            two lateral branches potentially forming at every node;            pinching enhances lateral branch development.        -   Plant height.—About 25 cm.        -   Plant diameter.—About 39 cm×about 34 cm.        -   Lateral branches.—Length: About 13 cm. Diameter: About            1.6 mm. Internode length: About 1.6 cm. Strength: Strong,            but flexible. Texture: Rough, pubescent. Color, young: Close            to yellow-green (RHS 144B). Color, woody: Closed to            greyed-brown (RHS 199A/B).        -   Stem.—Quantity of main branches per plant: About four to            five. Quantity of leaves per branch: About 12-13. Length of            stem: About 10-13 cm. Diameter: About 0.8-2.2 mm. Length of            internodes: About 1.0-2.4 cm. Texture: Pilose, and a few            glandular hairs on upper surface. Color: Close to yellow            green (RHS 144A and 144B).-   Foliage description:    -   -   Arrangement.—Opposite; simple.        -   Length.—About 5.2-8.4 cm.        -   Width.—About 3.1-6.2 cm.        -   Shape.—Ovate. Apex: Acute. Base: Obtuse with truncate            tendencies.        -   Margin.—Crenate to serrate.        -   Teeth along margins of leaf.—Mostly 34 to 44.        -   Texture, upper and lower surfaces.—Leathery, coarse,            pubescent.        -   Luster.—Upper surface: Slightly glossy with tiny hairs.            Lower surface: Dull.        -   Color, developing and fully expanded foliage.—Upper surface,            developing: Close to green (RHS 147A). Upper surface,            expanded: Close to green (RHS 147A). Lower surface,            developing: Close to green (RHS 147B). Lower surface,            expanded: Close to green (RHS 147B).        -   Venation pattern.—Arcuate.        -   Color of veins.—Upper surface: Close to green (RHS 146A).            Blends in with leaf color. Lower surface: Close to green            (RHS 146B/C).        -   Petiole.—Length: About 1.1-2.7 cm. Diameter: About            0.9-1.6 mm. Texture, both surfaces: Slightly pubescent.            Color: Upper surface: Close to yellow-green (RHS 146C).            Lower surface: Close to yellow-green (RHS 146D).-   Inflorescence description:    -   -   Flower type.—Umbel-like, flattened semi-sphere; flowers are            sessile on ovate receptacle.        -   Flowering habit.—Freely flowering, with potentially two            inflorescences per node; typically about 22-30 flowers per            umbel; flowering is continuous and consistent, spring until            front in the autumn; flowers are self-cleaning.        -   Flowering longevity on the plant.—About one week.        -   Fragrance.—None detected.        -   Inflorescence diameter.—About 4.5 cm.        -   Inflorescence height.—About 2.4 cm.        -   Number of flowers per inflorescence.—About 22-30.        -   Quantity of inflorescences per plant.—About 15; more during            the warm season.        -   Flowers (fully open).—Flower form and appearance: Flared            trumpet, corolla fused, four-parted; flowers roughly            rectangular in shape. Flower horizontal diameter: About 1.3            cm×about 1.1 cm. Flower length: About 2.1 cm.        -   Flower buds (before showing color).—Length: About 5.5 mm.            Diameter (width): About 1.3 mm. Shape: Corolla tubes are            roughly spherical to ovoid; Flower buds are nearly            rectangular when viewed from the top. Color: Close to            yellow-green (RHS 144D).        -   Bract.—Length: About 5.5 mm. Diameter: About 1.1 mm. Color:            Close to yellow-green (RHS 144B) near base and close to            green (RHS 143A) near tip. Texture: Outer surface: Hirsute.            Inner surface: Glandular hairs on inner surface.        -   Corolla.—Arrangement/appearance: Single whorl of four petals            fused into a flared trumpet. Tube length: About 1.4 cm.            Throat and tube texture: Outer surface: Pubescent/slightly            hirsute basally. Inner surface: Papillose. Tube color            (mature): Outer surface: Close to greyed red (RHS 179C).            Inner surface, throat: Close to greyed red (RHS 179D).        -   Petal.—Length from throat: Upper and lower petals: About            6.3 mm. Lateral petals: About 2.7 mm. Width: Upper and lower            petals: About 7.7 mm. Lateral petals: About 4.4 mm. Shape:            Spatulate to somewhat orbicular. Apex: Rounded. Margin:            Entire. Degree of lobation: Moderate. Petal lobe texture,            upper and lower surfaces: Smooth, velvety. Color: Petal            lobes, when opening (immature): Upper surface: Close to            yellow (RHS 13A). Toward the apex: Close to yellow (RHS            13C). Lower surface: Close to yellow (RHS 9B/C). Petal            lobes, fully opened (mature): Upper surface: Close to orange            red (RHS 34A). Toward the apex: Close to orange red (RHS            34B). Lower surface: Close to orange red (RHS 31C). Throat:            Close to yellow (RHS 9A). Tube: Close to orange (RHS 28D).        -   Calyx.—Number of sepals: 4 sepals fused to form calyx.            Length: About 2.5 mm. Width: About 1.7 mm. Shape:            Lanceolate. Apex: Acute. Base: Truncate. Texture: Lower            surface (inside): Pubescent. Color: Upper surface: Close to            yellow-green (RHS 144A). Lower surface: Close to            yellow-green (RHS 144A).        -   Peduncles.—Length: About 1.9 cm. Diameter: About 1 mm.            Angle: About 12 degrees from the stem. Strength: Flexible,            but strong. Texture: Pubescent. Color: Close to yellow-green            (RHS 144A).        -   Pedicels.—Not observed, flowers not stalked.        -   Stamens.—Quantity/arrangement: Four per flower, joined to a            floral tube. Length of filament: About 1.2 mm. Color of            filament: Close to yellow (RHS 13B).        -   Anther.—Shape: Oblong. Length: About 1.2 mm. Color: Poorly            developed.        -   Pistils.—Quantity: One per flower. Length: About 4.4 mm.            Stigma shape: Rounded. Stigma color: Close to yellow-green            (RHS 144C). Style color: Close to yellow-green (RHS 150D).            Ovary color: Close to yellow-green (RHS 144A).        -   Pollen.—Amount: Most pollen grains are aborted.            Approximately 98% of the observed pollen grains are not            viable, and viable pollen grains are rarely observed. Color:            Not able to document.        -   Fruit.—None or rarely observed.

Assessment of Female Fertility

Table 1 shows fruit production of the new Lantana cultivar ‘UF-1013-1’and two checks (‘UF-1013A-2A’ and ‘Pink Caprice’ (commercial cultivar,not patented)) in two replicated field trials in Florida.

The two field trials were conducted at the University of Florida (UF)'sGulf Coast Research and Education Center (GCREC) in Balm, Fla.(southwest Florida, USDA hardiness zone 9a, and AHS heat zone 10) and atUF's Indian River Research and Education Center (IRREC) in Ft. Pierce,Fla. (southeast Florida, USDA hardiness zone 9b, and AHS heat zone9-10). The experimental design used in the Balm trial was a randomizedcomplete block with three blocks and two plants per plot. Ground beds atthe GCREC were raised about 20 cm, fumigated with Pic-Clor 60™ (Trical,Inc., Hollister, Calif.; active ingredients 1,3-dichloropropene andchloropicrin) at 448 kg per hectare in February 2015, and covered withwhite-on-black plastic. The experimental design used in the Ft. Piercetrial was a randomized complete block with four blocks and single-plantplots. Ground beds at the IRREC were not fumigated but treated with apre-emergent herbicide (Sandea®, 75.0% ai (halosulfuron-methyl), GowanCompany, L.L.C., Yuma, Ariz.) at a rate of 0.1056 grams L⁻¹ and a 2%solution of glyphosate (Roundup WeatherMAX®, 48.8% ai, MonsantoTechnology LLC, Saint Louis, Mo.) and covered with black ground cover.Plants in the two trials were grown in full sun.

At each site, ‘Pink Caprice’ and ‘UF-1013A-2A’ were included ascontrols. ‘Pink Caprice’ is very prolific in fruit (and seed) production(Czarnecki et al., 2012), while ‘UF-1013A-2A’ is highly infertile (Denget al., 2017). In addition, 21 commercial cultivars with various levelsof male and female fertility were randomly placed in each block at bothsites.

Fruit production data were collected from each plant in the Balm and Ft.Pierce trials. The four data collections in Balm were made on 17 Aug.,14 Sep., 16 Oct., and 18 Nov., 2015, respectively. The four datacollections in Ft. Pierce were made on 12 Aug., 10 Sep., 14 Oct., and 11Nov. 2015, respectively. In each collection, 20 peduncles were randomlysampled from each plant; thus, approximately 120 peduncles were sampledfor each cultivar grown in Balm, and 80 peduncles were sampled for eachcultivar grown in Ft. Pierce. Fruit on all harvested peduncles werecounted, regardless of maturity. An analysis of variance and separationof mean fruit production values was conducted using JMP® Pro 13.2.0 (SASInstitute Inc., Cary, N.C.) to compare the fruit production of the newLantana cultivar ‘UF-1013-1’ with that of ‘UF-1013A-2A’ and ‘PinkCaprice’. Mean values with the same letter within columns in Table 1 arenot significantly different by the Tukey's HSD procedure at P<0.05.

As shown in Table 1 and reported previously (Deng et al., 2017), ‘PinkCaprice’ produced the largest number of fruit (drupes) among all thecultivars in the two replicated trials. Each peduncle had an average of7.941 drupes in Ft. Pierce and 10.313 drupes in Balm, averaged to 9.127drupes per peduncle across the two sites and four harvests. The numberof drupes per peduncle for the sterile cultivar ‘UF-1013A-2A’ rangedfrom 0 to 0.050 and averaged to 0.015 across the two sites over the 4months. The number of drupes the new Lantana cultivar TF-1013-1′produced per peduncle ranged from 0 to 0.038 and averaged to 0.009across two experimental sites and over 4 months. This level of fruitproduction in the new cultivar ‘UF-1013-1’ represented greater than 99%reduction from the fruit production of ‘Pink Caprice’.

Seed germination: Seeds were extracted from mature drupes collected fromthe above experiments. Seeds were cleaned, air-dried, and germinated. Asubsample of seeds of ‘Pink Caprice’ were sent to Mid-West Seed Servicesin Brookings, S.Dak., a commercial seed testing laboratory, for seedviability tests. The new Lantana cultivar ‘UF-1013-1’ and ‘UF-1013A-2A’produced few or no seeds at either site and were therefore not testedfor viability. Seeds of ‘Pink Caprice’ showed an average of 65.0%viability, germinated readily, with an average germination percentage of45.0% in 60 days. For ‘UF-1013A-2A’, no mature drupes were collectedfrom Balm or Ft. Pierce. For the new Lantana cultivar ‘UF-1013-1’, threemature drupes were collected from the Ft. Pierce trial over four months.Three seeds were extracted, but all were abnormal when visuallyexamined. Thus, there were no seeds from the new Lantana cultivar‘UF-1013-1’ and ‘UF-1013A-2A’ for seed viability or germination tests.

Female Fertility Index (FFI): Fruit (seed) production per peduncle andseed germination are the primary factors determining lantana' s femalefertility. Female fertility index (FFI) was calculated by multiplyingfruit production per peduncle and seed germination. The FFI for ‘PinkCaprice’ was 4.107. Because of the lack of seed germination data, it wasnot possible to calculate the FFI for the new Lantana cultivar‘UF-1013-1’. However, based on its triploidy and extremely low fruitproduction, it was expected that the FFI for the new Lantana cultivar‘UF-1013-1’ would be close to zero.

TABLE 1 Fruit per peduncle 8 to 21 weeks post Total Total Totaltransplanting (WPT) peduncles fruit mature fruit Average Expt. 8 12 1621 examined collected collected fruit per Site Cultivars Aug. 12 Sept. 9Oct. 7 Nov. 11 (no.) (no.) (no.) peduncle Balm New cultivar  0.008 b0.008 b    0 b    0 b 480 2 0  0.004 b (‘UF-1013-1’)  0.012 b 0.050 b 0.025 b    0 b 481 11 0  0.023 b ‘UF-1013A-2A’ Bloomify ™ Red; USPP29,292) ‘Pink Caprice’ 14.258 a 8.850 a 10.117 a 8.025 a 480 4,950 1,41610.313 a 8 12 17 21 Aug. 12 Sept. 10 Oct. 14 Nov. 11 Ft. New cultivar 0.013 b    0 b  0.038 b    0 b 320 4 3  0.013 b Pierce (‘UF-1013-1’)‘UF-1013A-2A’    0 b    0 b    0 b    0 b 320 0 0    0 b (Bloomify ™Red; USPP 29,292) ‘Pink Caprice’ 11.588 a 8.325 a  6.263 a 5.588 a 3202,541 1,832  7.941 a

Assessment of Pollen Stainability

Table 2 shows pollen stainability of the new Lantana cultivar‘UF-1013-1’ and two checks (‘UF-1013A-2A’ (Bloomify™ Red) and ‘PinkCaprice’) when their plants were grown in Balm and Ft. Pierce, Fla. infull sun in 2015. Two pollen staining experiments were conducted. InExperiment 1, newly opened flowers were collected from plants grown inBalm, Fla. in late July 2015, and anthers were extracted from theflowers and collected into 1.5-mL Eppendorf tubes. The collected antherswere stained with 10⁻⁶ M fluorescein diacetate (FDA) (Sigma-Aldrich, St.Louis, Mo.) in 0.22 M sucrose at room temperature in the dark for 1 hour(Czarnecki et al., 2014). Stained anthers were transferred onto amicroscope slide and covered with a coverslip. Pollen grains in theanthers were released by gently tapping and pressing the coverslip andthen examined under a fluorescent microscope. Plump, round pollen grainsfluorescing bright yellowish green light were considered stainable,while misshaped, non-fluorescing, or unevenly, lightly fluorescingpollen grains were counted as non-stainable. In Experiment 2, flowerswere collected from lantana plants grown in Ft. Pierce, Fla. inmid-August 2015. Anther staining and pollen examination were performedas described in Experiment 1. The number of pollen grains examined foreach lantana cultivar in each staining experiment was between 1,094 and2,122. Pollen stainability data (in percentage) were arcsine-transformedbefore analysis of variance was performed. Means with the same letterwithin the column of Table 2 are not significantly different by the LSDprocedure at P<0.05. The analysis of variance and mean separation wereconducted using the software JMP® Pro 13.2.0.

As shown in Table 2, the average pollen stainability of the new Lantanacultivar ‘UF-1013-1’ was 2.2%, comparable to the average pollenstainability of sterile cultivar ‘UF-1013A-2A’. The average pollenstainability of ‘Pink Caprice’ was 73.1%. The pollen stainability (ormale fertility) of the new Lantana cultivar ‘UF-1013-1’ was reducedsubstantially (95%) from that of ‘Pink Caprice’.

TABLE 2 Pollen grains examined (no.) Pollen stainability (%) CultivarExperiment 1 Experiment 2 Experiment 1 Experiment 2 Average ‘UF-1013-1’1464 1840  2.0 b  2.4 b  2.2 b ‘UF-1013A-2A’ 2122 1466  1.5 b  4.5 b 3.0 b (Bloomify ™ Red; USPP 29,292) ‘Pink Caprice’ 1271 1094 70.8 a75.3 a 73.1 aAssessment of Hybridization Potential With Lantana depressa

Table 3 shows the hybridization potential of the new Lantana cultivar‘UF-1013-1’ with L. depressa as compared to ‘UF-1013A-2A’ and ‘PinkCaprice’.

Hand pollination experiments were performed in a greenhouse at GCREC inBalm, Fla. in June and July 2015 to assess the hybridization potentialof the new Lantana cultivar ‘UF-1013-1’, as a male or female parent,with L. depressa. ‘UF-1013A-2A’ and ‘Pink Caprice’ were included in thepollination experiments as an infertile and a fertile check,respectively. Stock plants of all lantana cultivars and L. depressa weregrown in 1-gallon plastic containers and arranged into three blocks andin each block, they were randomly placed on the benches. Theexperimental unit consisted of two containerized plants. Air temperatureinside the greenhouse was from 21° C. to 33° C. No supplemental lightingwas provided. Plants were drip-irrigated twice per day. Fresh antherswere collected from mature unopened flowers of male parents and appliedimmediately to emasculated flowers of female parents. At maturity, fruitproduced by the pollinated flowers were collected and counted, and seedswere extracted and sown to determine seedling emergence.

Fruit set data (in percentage) were arcsine-transformed before analysisof variance was performed in JMP® Pro 13.2.0. Mean values with the sameletter within the column are not significantly different by the LSDprocedure at P<0.05.

As shown in Table 3, ‘Pink Caprice’, as a male parent, caused an averageof 8.6% fruit set on L. depressa. When pollinated with L. depressa,‘Pink Caprice’ flowers showed 19.9% fruit set. Seeds from crossesbetween ‘Pink Caprice’ and L. depressa or vice versa showed 11.1% or15.8% seedling emergence. As a male parent, ‘UF-1013A-2A’ did not causeany fruit set on L. depressa flowers, nor did it set any fruit afterhand-pollination with L. depressa. A total of 389 L. depressa flowerswere pollinated with the new cultivar, and none of the pollinatedflowers set fruit, resulting in 0% fruit set (Table 3). When the newcultivar was used as the female parent, it did not set any fruit afterit was hand-pollinated with L. depressa. Thus, the new cultivar did nothybridize with L. depressa (Table 3). These data confirm the high levelof male and female infertility in the new cultivar.

TABLE 3 L. depressa as the female parent L. depressa as the male parentFlowers Fruit set Seedling Flowers Fruit set Seedling Cultivarpollinated (no.) (%) emergence (%) pollinated (no.) (%) emergence (%)‘UF-1013-1’ 389  0 b — 496   0 b — ‘UF-1013A-2A’ 353  0 b — 558   0 b —Deng et al. (2017) (Bloomify ™ Red; USPP 29,292) ‘Pink Caprice’ 388 8.6a 11.1 452 19.9 a 15.8 Deng et al. (2017)

Assessment of Nuclear DNA Contents

As shown in Table 4, the new cultivar ‘UF-1013-1’ has an average nuclearDNA content of 4.82 pg/2C and is a triploid. The nuclear DNA content wasdetermined using a CyFlow® Cube 6 flow cytometer (Sysmex Partec GmbH,Munster, Germany) and the procedure described by Dolz̆el et al.(Estimation of Nuclear DNA Content in Plants Using Flow Cytometry, Nat.Protoc. 2:2233-2244 (2007)) and modified by Cao et al. (2014). Peacultivar ‘Ctirad’ (Pisum sativum) with a nuclear content of 9.09 pg/2Cwas used as the internal reference for this determination in this study.The new cultivar ‘UF-1013-1’ has 6.2% higher nuclear DNA content than‘UF-1013A-2A’ does (4.82 pg/2C vs. 4.54 pg/2C). The ploidy level of‘UF-1013-1’ was determined by comparing its nuclear DNA content with theDNA content of known diploid, triploid and tetraploid lantana cultivars.

TABLE 4 Mean nuclear DNA Standard Cultivars content (pg/2C) deviation(SD) Ploidy level ‘UF-1013-1’ 4.82 0.11 3× ‘UF-1013A-2A’ 4.54 0.08 3×(Bloomify ™ Red; USPP 29,292) ‘Pink Caprice’ 6.25 0.17 4×

DNA Fingerprints

As shown in Table 5, the new cultivar ‘UF-1013-1’ carries two alleles(152 and 160 base pairs (bp)) at the Lantana11 SSR marker locus, threealleles (135, 143 and 147 bp) at the Lantana12 SSR marker locus, and oneallele (93 bp) at the Lantana20 SSR marker locus.

This DNA fingerprint analysis was performed using three pairs oflantana-specific SSR primers. Lantana genomic DNA was isolated fromlantana leaves in Balm, Fla. Primers were developed as described by Gongand Deng (Development and Characterization of Microsatellite Markers forCaladiums (Caladium Vent.), Plant Breeding 130(5) (2011)) fromSSR-enriched lantana genomic sequences (Gong and Deng, unpublished).Nucleotide sequences of the three pairs of primers are: Lantana11F: (M13tail sequence)-TGCAATTGGAGGCTTTTTCT, and Lantana11R:AAAGCAGCTTCAAGTTTGTGC; Lantana12F: (M13 tailsequence)-GGATGAGATGATAAGGTAGGGTGT, and Lantana12R:TTGGTGGTGATGACTTTGATTC. Lantana20F: (M13 tailsequence)-AGAATCAGGGTTTGGGGTTG, and Lantana20R: TCGTAGCCACCACTCCTCAC.The M13 tail sequence was 5′-CCCAGTCACGACGTTG-3′. Polymerase chainreaction (PCR) amplification, capillary electrophoresis, and allelescoring were performed at the United State Department ofAgriculture/Agricultural Research Services Fruit and Tree Nut ResearchLaboratory, Byron, Ga., using a procedure previously described by Chenet al. (Genome-wide Characterization and Selection of Expressed SequenceTag Simple Sequence Repeat Primers for Optimized Marker Distribution andReliability in Peach, Tree Genet. Genome 10:1271-1279 (2014)) with minormodifications.

As shown in Table 5, the female parent of the new cultivar, breedingline DROP-25, carries three alleles (150, 152 and 160 bp) at theLantana11 marker locus, four alleles (135, 143, 145 and 147 bp) at theLantana12 marker locus, and two alleles (93 and 109 bp) at the Lantana20marker locus.

TABLE 5 Alleles amplified by SSR markers (size of alleles in base pairs)Lantana Marker Lantana11 Marker Lantana12 Lantana20 cultivars 150 152156 160 135 143 145 147 150 152 93 109 New cultivar + + + + + +(‘UF-1013-1’) ‘UF-1013A-2A’ + + + + + + (Bloomify ™ Red; USPP 29,292)DROP-25 + + + + + + + + + ‘Pink Caprice’ + + + + + + + + + “+” indicatesthe presence of the respective alleles in the cultivars.

I claim:
 1. A new and distinct Lantana camara plant named ‘UF-1013-1’,as illustrated and described herein.